Relationship between the injury time and DNA fragmentation and its quantity in rat brain.
- Author:
Fan LI
1
;
Zhi-Gang LIAO
;
Shu-Ling MA
;
Ye WANG
;
Peng GUAN
;
Min LIU
Author Information
1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. fanlfy@mail.haust.edu.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Apoptosis;
Brain/pathology*;
Brain Injuries/pathology*;
DNA/analysis*;
DNA Fragmentation;
Disease Models, Animal;
Forensic Pathology;
In Situ Nick-End Labeling/methods*;
Male;
Neurons/pathology*;
Random Allocation;
Rats;
Rats, Wistar;
Staining and Labeling/methods*;
Time Factors
- From:
Journal of Forensic Medicine
2006;22(3):168-170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the changes of DNA fragmentation and its quantity along with the time of injury in nerve cells after brain contusion.
METHODS:The model of brain contusion caused by free drop hammer was established. TUNEL and Feulgen's DNA staining conjoined with image analysis technique were used for exploration.
RESULTS:With the gradually rising of DNA fragmentation, DNA quantity was declining in the brain tissue after contusion.
CONCLUSION:TUNEL and Feulgen's DNA staining conjoined with image analysis technique could be utilized in the timing of brain injury and provide a new approach for this issue.
