Study on colloidal gold labeled anti-buprenorphine monoclonal antibody for rapid test kit to detect buprenorphine.
- Author:
Li-Bo ZENG
1
;
Lian-Kang CHEN
;
Xiao-Long HU
;
Liang CHEN
;
Xue-Sheng WANG
;
Yu-Rong ZHANG
Author Information
1. Shanghai Forensic Science and Technology Research Institute, Shanghai 200083, China.
- Publication Type:Journal Article
- MeSH:
Analgesics, Opioid/urine*;
Antibodies, Monoclonal/immunology*;
Buprenorphine/urine*;
Enzyme-Linked Immunosorbent Assay/methods*;
Gold Colloid;
Humans;
Reagent Kits, Diagnostic;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2006;22(2):130-132
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develope an easy to use, rapid and accurate test for detecting buprenorphine based on the principle of competitive immunoassay.
METHODS:Monoclonal antibody against buprenorphine was conjugated with colloidal gold and dispensed on the glass fiber. The complete antigen Buprenorphine-BSA and the goat anti-mouse IgG polyclonal antibody were separately sprayed on the nitrocellulose membrane as the test line (T line) and the control line (C line). The rapid test kit was the final assembled product of test strip with the plastic cover.
RESULTS:A total of 100 urine samples were tested for buprenorphine by immunochromatographic and GC/ MS methods. The accuracy was 99.0%. It is found the test kit can only detect by cross reaction with other 45 kind drugs.
CONCLUSION:Rapid test kit can detect buprenorphine in the samples in 5 minutes. The cut-off value of the test is 100 ng/mL.
