The application of PCR-SSCP in forensic mtDNA typing.
- Author:
Hong-Wei AI
1
;
Chun-Ying YU
;
Gui-Sen ZHAO
;
Dai-Xin HUANG
;
Ji KE
;
Xia YUE
;
Qing-En YANG
Author Information
1. Department of Forensic Medicine, Huazhong University of Science and Technology, Wuhan 430030, China. aimingxu03@163.com
- Publication Type:Journal Article
- MeSH:
DNA Fingerprinting/methods*;
DNA Primers;
DNA, Mitochondrial/genetics*;
Forensic Genetics/methods*;
Haplotypes;
Humans;
Pedigree;
Polymerase Chain Reaction/methods*;
Polymorphism, Single-Stranded Conformational;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2006;22(2):117-119
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the application of PCR-SSCP in forensic mtDNA typing.
METHODS:Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.
RESULTS:In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD = 0.9556; 16 haplotypes were found in HVII, GD = 0.9356.
CONCLUSION:PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects.
