Developing of a new multicolor-fluorescent labeled STR amplification kit.
- Author:
Li LI
1
;
Guang-Hui CHEN
;
Cheng-Tao LI
;
Yan LIU
;
Yuan LIN
;
Ting-Zhi QUE
;
Zhen-Min ZHAO
;
Ya-Xin WANG
Author Information
1. Institute of Forensic Sciences, Ministry of Justice, P.R. China, Shanghai 200063, China. anneinchina@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
Animals;
DNA Fingerprinting/methods*;
DNA Primers;
Forensic Genetics;
Gene Frequency;
Genotype;
Humans;
Indicators and Reagents;
Polymerase Chain Reaction/methods*;
Polymorphism, Genetic;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2006;22(2):111-116
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253).
METHODS:By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci.
RESULTS:Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit.
CONCLUSION:The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.
