Identification of six species of sarcosaphagous flies (Diptera) by sequence analysis of cytochrome oxidase subunit I gene (COI) in Weifang.
- Author:
Xin-Jie WANG
1
;
Xue-Hai WANG
;
Li-Jiang DIAO
;
Gui-Ping LU
Author Information
1. Public Security Bureau of Weifang municipality, Weifang 261061, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
China;
DNA Primers;
DNA, Mitochondrial/genetics*;
Diptera/genetics*;
Electron Transport Complex IV/genetics*;
Forensic Medicine;
Genes, Insect;
Humans;
Larva/genetics*;
Phylogeny;
Polymerase Chain Reaction/methods*;
Pupa/genetics*;
Sequence Analysis, DNA/methods*;
Species Specificity
- From:
Journal of Forensic Medicine
2006;22(2):93-94
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify sarcosaphagous flies and their larvae, pupa.
METHODS:Sarcosaphagous flies and their larvae, pupas were collected from human corpses and their surroundings in the Weifang city. A 304 bp region in COI gene was analyzed by mtDNA sequencing.
RESULTS:The studied region showed no sequence divergence within same species and significant difference were found between different species in all samples.
CONCLUSION:It is a practical approach to identify these Sarcosaphagous flies and their larvae, pupas by sequence analysis of the 304bp region of the COI in mtDNA.
