Determination of opiates in biological human samples by liquid chromatography-tandem mass spectrometry.
- Author:
Ping XIANG
1
;
Min SHEN
;
Bao-hua SHEN
;
Dong MA
;
Jun BU
;
Yan JIANG
;
Xian-yi ZHUO
Author Information
1. Institute of Forensic Sciences, Ministry of Justice, P.R.China.
- Publication Type:Journal Article
- MeSH:
Chromatography, Liquid;
Codeine/analysis*;
Forensic Medicine/methods*;
Hair/chemistry*;
Humans;
Hydrocodone/analysis*;
Hydromorphone/analysis*;
Morphine/analysis*;
Narcotics/analysis*;
Reproducibility of Results;
Saliva/chemistry*;
Substance Abuse Detection/methods*;
Tandem Mass Spectrometry
- From:
Journal of Forensic Medicine
2006;22(1):52-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of opiates in biological samples according to the emerging problem in drugs abuse.
METHODS:Opiates such as heroin, 6-acetylmorphine, morphine, codeine, acetylcodeine, hydrocodone and hydromorphone were isolated from human blood, urine, oral fluid and hair using a simple extraction and consequently analyzed using LC-MS/MS. The method was evaluated by real cases.
RESULTS:The mobile phase give the optimum separation for opiates. The detection limit of morphine in urine with dilution and liquid-liquid extraction and in hair is 10ng/mL, 0.01 ng/mL and 0.01 ng/mg, respectively.
CONCLUSION:The method is simple and rapid, offering superior sensitivity and selectivity for opiates. The target compounds comprising hydrocodone and hydromorphone enlarge the applied area.
