Study on application of the whole genome amplification in LCN.
- Author:
Huai-gu ZHOU
1
;
Chen ZHANG
Author Information
1. Shanghai Institute of Criminal Science and Technology, Shanghai 200083,China.
- Publication Type:Journal Article
- MeSH:
DNA/analysis*;
DNA Fingerprinting/methods*;
Genome, Human;
Genotype;
Humans;
Microsatellite Repeats;
Nucleic Acid Amplification Techniques/methods*
- From:
Journal of Forensic Medicine
2006;22(1):43-47
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for whole genome amplification (WGA) based on (multiple displacement amplification MDA), and achieve DNA analysis of the human genome from low copy number (LCN).
METHODS:DNA sample was used for WGA according to the REPLI-g kit protocol (QIAGEN, Germany). WGA product was used for DNA analysis according to the Applied Biosystems Profiler Plus kit protocol (ABI, USA).
RESULTS:WGA product of DNA sample (10 pg) can be used for STR genotyping.
CONCLUSION:WGA technology could be helpful for LCN DNA analysis.
