Constructing of STR slippage model by optimizing some factors.
- Author:
Jian-qiang DENG
1
;
Ying-bi LI
;
Jin WU
;
Yi-ping HOU
Author Information
1. Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063,China. forensicpatho@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
DNA/isolation & purification*;
DNA Primers;
Genetic Markers;
Genetic Variation;
Genotype;
Humans;
Magnesium;
Models, Genetic;
Polymerase Chain Reaction/methods*;
Tandem Repeat Sequences/genetics*
- From:
Journal of Forensic Medicine
2006;22(1):39-42
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct STR slippage model and study factors involved in this procedure.
METHODS:DNA samples were amplified with the technology of Degenerate oligonucleotide- primed PCR, then their products were taken as later DNA template and their STR genotype were analyzed by optimizing several factors.
RESULTS:STR slippage model was constructed.
CONCLUSION:Several factors were involved in the produce of STR slippage, such as amount of modulate DNA, concentration of MgCl2, property of DNA polymerase, motif sequence of STR loci, sample, etc.
