Identification of sarcosaphagous flies by analyses the sequence of 16S rDNA.
- Author:
Xiao-ming SUN
1
;
Ji-feng CAI
;
Bin-wu YING
;
Yue-qin FANG
;
Li-bing YUN
;
Wan-an YUAN
;
Shan GAO
;
Yi-ping HOU
Author Information
1. Institute of Forensic Medicine, Sichuan University, Chengdu 610041, China. lupusstar@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
DNA, Mitochondrial/genetics*;
DNA, Ribosomal/genetics*;
Diptera/genetics*;
Forensic Medicine/methods*;
Molecular Sequence Data;
Polymerase Chain Reaction/methods*;
RNA, Ribosomal, 16S/genetics*;
Rabbits;
Sequence Analysis, DNA;
Species Specificity
- From:
Journal of Forensic Medicine
2006;22(1):36-38
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To solve the difficulties of identification of Sarcosaphagous flies such as Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) which could not be identified by analyzing the 278bp and 635 bp regions of the gene encoding for cytochrome oxidase subunit I and II (CO I and CO II) in mtDNA.
METHODS:Specimens were collected from the corpses of rabbits on the grassland in Huhhot and Chengdu, the sequences of 551 bp region of 16S rDNA of their mtDNA were analyzed, the multiple-alignment program DNAMAN(version 4.0) and MEGA 2.1 sofeware were employed for sequence alignments neighbour-joining tree construction.
RESULTS:Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) were distinguished successfully by sequence analysis of The 551 bp region of the gene of 16S rDNA.
CONCLUSION:The 551 bp region of the gene of 16S rDNA of sarcosaphagous flies can be used for identifying them on species level effectively. It is likely to be a successful compliment to identify the sarcosaphagous flies by sequence analysis of CO I and CO II in mtDNA.
