Species identification of biological samples by amplifying intron 8 of the TP53 gene.
- Author:
Chuang WANG
1
;
Lin ZHANG
;
Bin ZHOU
Author Information
1. Department of Forensic Biology, College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Blood Stains;
Cats;
Cattle;
Chickens;
DNA/genetics*;
DNA Primers;
Dogs;
Electrophoresis, Polyacrylamide Gel;
Fishes;
Forensic Medicine;
Gene Amplification;
Genes, p53;
Humans;
Introns/genetics*;
Mice;
Polymerase Chain Reaction/methods*;
Rabbits;
Species Specificity;
Staining and Labeling
- From:
Journal of Forensic Medicine
2005;21(3):195-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify the species of biological samples by amplifying intron 8 of the TP53 gene.
METHODS:Collected the bloodstains or muscle tissues of 14 kinds of common animals and human. DNA were quantified after extraction. The PCR amplification products were analyzed by PAGE and silver staining.
RESULTS:The human and monkey have one amplification band of 460 bp while the eel, fish, frog, duck, rabbit, cat, mouse, cavy, pig, ox and sheep could amplify different unspecial bands. No amplification products were found in chicken and dog.
CONCLUSION:The method of species identification by amplifying intron 8 of the TP53 gene is simple and sensitive.
