Expression of c-Fos in rats organs after electrical injury.
- Author:
Ye WANG
1
;
Zhi-gang LIAO
;
Shi-chun WANG
Author Information
1. West China Preclinical & Forensic Medicine School of Sichuan Uiversity, Chengdu, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain/pathology*;
Death;
Disease Models, Animal;
Electric Injuries/pathology*;
Immunohistochemistry;
Kidney/metabolism*;
Liver/metabolism*;
Lung/pathology*;
Male;
Myocardium/pathology*;
Proto-Oncogene Proteins c-fos/metabolism*;
Rats;
Rats, Sprague-Dawley;
Staining and Labeling;
Time Factors
- From:
Journal of Forensic Medicine
2005;21(3):171-176
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression of c-fos in organs after rats electrified.
METHODS:The brain, lung, heart, liver, spleen, kidney, muscle of electrified limb, and cutis of electrified limb of all experimental rats and those organs of control groups were dissected to detect the expression of c-fos by using immunohistochemistry staining, and the staining intensity were assessed by image analysis system.
RESULTS:The expression of c-fos was observed in brain, heart, liver, lung, kidney and muscle in electrified directly group, the amount of expression reached peak at 2 h after electrified and decreasing at 8 h after electrified, and the expression showed faintness in electrified at the immediate after death group. The expressions of c-fos in spleen and cutis is negative in all groups. The expression of c-fos in all detected organs was negative in other rats that were electrified after death.
CONCLUSION:c-fos can be regard as a target in identifying electrical injury, whether it formed at antemortem or postmortem.
