Ion-pair solid-phase extraction (SPE) and HPLC analysis of paraquat in biological sample.
- Author:
Rui-hua WANG
1
;
Shao-ming SU
;
Guang-ming QIN
Author Information
1. Forensic Medicine School of Kunming Medical College, Kunming 650031, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Chromatography, High Pressure Liquid/methods*;
Herbicides/chemistry*;
Liver/chemistry*;
Paraquat/analysis*;
Rabbits;
Sensitivity and Specificity;
Sodium Dodecyl Sulfate;
Solvents;
Swine
- From:
Journal of Forensic Medicine
2005;21(2):121-123
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of Paraquat in biological samples.
METHODS:Paraquat in biological samples was extracted by C18 columns which were pre-treated with cetyl-trimethyl ammonium bromide (CTAB) and soudium dodecyl sulphate (SDS), and analysed by HPLC/DAD.
RESULTS:The detection limit of the method was 1 ng x mL(-1), and the average recoveries were 81%-94%.
CONCLUSION:The method can be used to analysis of paraquat in biological samples.
