Study on identification of aconiti by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
- Author:
Ai-hua PAN
1
;
Jie LIU
Author Information
1. Department of Forensic Toxicological Analysis, Preclinical School, Sun Yat-Sen University, Guangzhou 510089, China.
- Publication Type:Journal Article
- MeSH:
Aconitine/analysis*;
Aconitum/chemistry*;
Drugs, Chinese Herbal/isolation & purification*;
Ethanol;
Methylene Chloride;
Plant Roots/chemistry*;
Plants, Medicinal/chemistry*;
Quality Control;
Sensitivity and Specificity;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*;
Technology, Pharmaceutical/methods*
- From:
Journal of Forensic Medicine
2005;21(2):118-120
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The merhod of determination of traditional Chinese medicine of aconitum root has been established by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
METHODS:With optimized matrix of 2,5-Dihydroxy- benzoic acid for herbal extract, the identification of aconitum root by MALDI-TOF-MS has been conditioned. By comparison of mass spetra of various of aconitium root, their fingerprint mass-to-charge ratios were found to perform identification. With the characteristic mass-to-charge ratios, It was shown that the mass spectrum of raw aconitum roots were obviously different from that of processed aconitum roots.
RESULTS:The raw radix aconiti could be differentiated from radix aconiti preparate by determination of MALDI-TOF-MS.
CONCLUSION:The identifical method of aconitum root by MALDI-TOF-MS is rapid, precise and sensitive, it can be used in toxicological analysis of medical tangle and illegal medical practice.
