Estimation of postmortem intervals by using terminal deoxynucleotidyl transferase.
- Author:
Lan ZHAN
1
;
Zi-qin ZHAO
;
Yi-wen SHAN
Author Information
1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Nucleus/metabolism*;
Clinical Enzyme Tests/methods*;
DNA/metabolism*;
DNA Nucleotidylexotransferase/metabolism*;
Kidney/metabolism*;
Liver/metabolism*;
Male;
Postmortem Changes;
Pyrophosphatases/metabolism*;
Rats;
Rats, Sprague-Dawley;
Spectrophotometry, Ultraviolet;
Spleen/metabolism*;
Time Factors
- From:
Journal of Forensic Medicine
2005;21(2):113-114
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between DNA degradeation and postmortem interval.
METHODS:dUTP was transferred to 3'terminal of DNA by using terminal deoxynucleotidyl transferase (TDT), then the reminders of dUTP after experimental reaction, as indicator of quantity of DNA degradation, were detected.
RESULTS:The reminders of dUTP were decreasing along with the postmortem interval.
CONCLUSION:Postmortem DNA degradation may be used in postmortem interval judgment.
