Forensic applications of the sequencing of mitochondrial DNA cytochrome oxidase subunit I gene for sarcosaphagous flies (Diptera) in Huhhot and Dunhuang district.
- Author:
Ji-feng CAI
1
;
Jian-guo DONG
;
Min LIU
Author Information
1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. cjf_jifeng@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Base Sequence;
DNA, Mitochondrial/genetics*;
Diptera/genetics*;
Electron Transport Complex IV/genetics*;
Forensic Medicine/methods*;
Larva/genetics*;
Phylogeny;
Polymerase Chain Reaction/methods*;
Pupa/genetics*;
Rabbits;
Sequence Analysis, DNA;
Species Specificity;
Swine
- From:
Journal of Forensic Medicine
2005;21(2):100-106
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To solve the problems of identification of Sarcosaphagous flies and their larvae, pupas and eggs.
METHODS:Sarcosaphagous Flies (Diptera) Samples were collected on the corpses of rabbits in the Huhhot district and a pig in the Dunhuang district. A 278bp region in the cytochrome oxidase subunit I (CO I) gene in mtDNA was analysed by DNA sequencing, A neighbour-joining tree using the Tamura and Nei model of nucleotide substitution was also constructed using the MEGA2.1 package.
RESULTS:A 278 base pairs region of the gene for CO I encoding region of mtDNA of above all samples was showed less than 1% sequence divergence within species and about 3% divergence between species.
CONCLUSION:It is an effective, easy and accurate method to be used for identification of these Sarcosaphagous Flies (Diptera) to species group by sequencing the 278 base pairs region of the CO I encoding gene of mtDNA.
