SNP genotyping by multiplex amplification and microarrays assay and forensic application.
- Author:
Li LI
1
;
Rong-yu LI
;
Cheng-tao LI
Author Information
1. Institute of Forensic Sciences, Ministry of Justice, PR China, Shanghai 200063, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Asian People/genetics*;
DNA Fingerprinting/methods*;
DNA Primers;
Forensic Medicine/methods*;
Genotype;
Humans;
Oligonucleotide Array Sequence Analysis;
Paternity;
Polymerase Chain Reaction;
Polymorphism, Single Nucleotide;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2005;21(2):90-95
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Research on the application feasibility of SNP genotyping for forensic identification by microarrays.
METHODS:Oligonucleotide microarrays which could detect 31 different SNPs were used. Population studies on 31 SNP loci was carried out in a sample of 109 unrelated Chinese Han individuals using oligonucleotide microarrays for genotype detection. The method was also applied to cases.
RESULTS:No deviations from Hardy-Weinberg equilibrium could be found at the 31 SNP loci 4 loci were medium informative and 27 were high informative. The combination discrimination power (CDP) of the 31 optimal informative SNPs was 0.9999999999979. The matching probability was 2.13 x 10(-12). The average exclusion probability in duos and trios were 0.9609 and 0.9970 respectively.
CONCLUSION:The data and case application demonstrated that SNP typing by oligonucleotide probe microarrays was a useful technique for paternity testing and individual identification.
