The study of the sequence of molecular markers of mitochondrial DNA of Sarcosaphagous Flies.
- Author:
Ji-feng CAI
1
;
Bin-wu YING
;
Tao TAO
Author Information
1. Forensic Pathology Department, School of Legal Medicine, Sichuan University, Chengdu 610041, China.
- Publication Type:Review
- MeSH:
Animals;
DNA, Mitochondrial/genetics*;
Diptera/genetics*;
Electron Transport Complex IV/genetics*;
Forensic Medicine/methods*;
Humans;
Larva/genetics*;
Polymerase Chain Reaction/methods*;
Sequence Analysis, DNA;
Species Specificity
- From:
Journal of Forensic Medicine
2005;21(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
Identifying sarcosaphagous flies specimens is an important first step in a forensic-entomological analysis. It is traditionally performed using morphological features of the Sarcosaphagous Flies. However, Morphological identification may be complicated by the numerical diversity of species and physical similarity between different species, particularly in immature stages. The sequences focused on some sections of the cytochrome oxidase I and II (CO I and CO II) encoding region of mtDNA could be as the prospective basis of a diagnostic technique. By Analysis of these sequences, forensic doctors can reveal abundant phylogenetic informative nucleotide substitutions that could effectively identify Sarcosaphagous flies to species group. It was not reported in our country before and was reviewed in this article now.
