Primarily study and significance of ET-1 during ischemia with RT-PCR.
- Author:
Meng-yun MA
1
;
Xiao-hu XU
Author Information
1. Shenzhen Institute of Criminal Science and Technology, Shenzhen 518001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Endothelin-1/genetics*;
Female;
Image Processing, Computer-Assisted;
Male;
Myocardial Ischemia/metabolism*;
Myocardium/pathology*;
RNA, Messenger/biosynthesis*;
Rats;
Rats, Sprague-Dawley;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Forensic Medicine
2005;21(1):21-26
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the relationship between expression of endothelin in heart and ischemia.
METHODS:Using the RT-PCR method, we enquiry changes of ET-1 RNA after 60min ischemia in hearts of rats.
RESULTS:Although the two group appear positive, they are different significantly, which indicate that ischemia may lead to the increase of ET-1 mRNA.
CONCLUSION:The experiment, therefore, pave a way for immunochemical study.
