A preliminary study on estimation of postmortem interval according to beta-actin mRNA stability in rat.
- Author:
Jun-hui XIAO
1
;
Yu-chuan CHEN
;
Jiang-feng WANG
Author Information
1. Department of forensic pathology, preliminary medical college of Sun Yat-Sen University, Guangzhou 510080, China. xiaoxiaopost@163.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Actins/genetics*;
Animals;
Forensic Medicine/methods*;
Lung/metabolism*;
Male;
Pectoralis Muscles/metabolism*;
Postmortem Changes;
RNA, Messenger/metabolism*;
Rats;
Rats, Sprague-Dawley;
Reverse Transcriptase Polymerase Chain Reaction;
Time Factors
- From:
Journal of Forensic Medicine
2005;21(1):19-20
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:In order to find a new parameter to estimate the postmortem interval, beta-actin mRNA in lung and thoracic muscle of rats was detected at different time point postmortem.
METHODS:Rats were killed by neck dislocation and left in a temperature controlling system at 21 degrees C for 12 days postmortem. Total RNA in lung and thoracic muscle at different time point was extracted and beta-actin mRNA expression was examined by RT-PCR. Semi-quantification analysis of the image of electrophoresis was performed to confirm the changes of beta-actin mRNA expression.
RESULTS:beta-actin mRNA in lung of rats still could be detected at 12 days postmortem, but-disappeared in thoracic muscle at 8 days postmortem.
CONCLUSION:The expression of beta-actin mRNA in lung and thoracic muscle could be a new parameter for estimation of PMI.
