Detecting haplotypes of four Y-STR by multiplex amplification.
- Author:
Qiu-ling LIU
1
;
De-jian LU
;
Ni-Mei XU
Author Information
1. Faulty of Forensic Medicine, Zhong shan Medical College, Zhong shan University, Guangzhou 510089, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
China;
Chromosomes, Human, Y;
DNA/isolation & purification*;
Electrophoresis, Polyacrylamide Gel;
Ethnicity/genetics*;
Female;
Forensic Medicine;
Gene Frequency;
Haplotypes;
Humans;
Male;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Silver Staining;
Tandem Repeat Sequences/genetics*
- From:
Journal of Forensic Medicine
2005;21(1):9-14
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population.
METHODS:DYS439, DYS390, GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining.
RESULTS:When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439, DYS390, GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853.
CONCLUSION:The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.
