DNA samples preparation from single cell and its application in sensitivity test.
- Author:
Jian-qiang DENG
1
;
Mei-sen SHI
;
Bing-wu YING
Author Information
1. Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China,
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
DNA/isolation & purification*;
DNA Fingerprinting/methods*;
Microscopy;
Polymerase Chain Reaction;
Sensitivity and Specificity;
Specimen Handling/methods*;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2005;21(1):6-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a reliable, exact and practical method to prepare DNA samples for sensitivity-test purposes.
METHODS:The micromanipulation method was employed to prepare exact quantity DNA samples used to study the sensitivity of Profiler Plus Kit-ABI PRISM 310 system.
RESULTS:We succeed in establishing a micromanipulation method to prepare groups of DNA samples, which contain 1-11 cells in turn, and also succeed in using them to study the sensitivity of Profiler Plus Kit-ABI310 system.
CONCLUSION:The method we have established is proved to be a reliable, exact and practical way to prepare DNA samples for sensitivity-test purposes.
