A study of the expression of Fas after fluid percussion brain injury in rats.
- Author:
Hong-fu PAN
1
;
Ye WANG
;
Xiao-gang CHEN
;
Jing YANG
;
Leibo LI
;
Qiyi PENG
;
Wendong WANG
;
Min LIU
Author Information
1. West China College of Basic Medicine and Forensic Medicine of Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Brain/metabolism*;
Brain Injuries/pathology*;
Fas Ligand Protein;
Image Processing, Computer-Assisted;
Immunohistochemistry;
Male;
Membrane Glycoproteins/biosynthesis*;
Rats;
Rats, Wistar;
Time Factors;
Tumor Necrosis Factors/biosynthesis*
- From:
Journal of Forensic Medicine
2004;20(4):205-207
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the pathologic diagnosis and the injury time estimation in light closed encephalon injury.
METHODS:Mice were hurt by fluid percussion, and were killed at 15, 30 min, 1, 3 , 6, 12 h, 1, 4, 7, 14 d respectively after injury. The expression of Fas-L in the cerebral cortex, thalamus, and hippocampi was detected by immunohistochemistry and the results were assessed by image analysis system.
RESULTS:It is showed that the expression of Fas-L could be detected in 1 h after injury, and increased significantly in three hours, and it reached apex 12 h after injury, and decreased gradually four days after injury, and returned normal 14 days after injury.
CONCLUSION:This research demonstrated that Fas-L mediated apoptosis appeared not only around brain trauma but also in the brain tissue far away from the traumatic area. It indicted that the expression of Fas-L is a useful target for diagnosis of early brain injury; the regularity of Fas-L expression could be used as one of indication to date the time of brain injury.
