A sort of meliorative method for testing DNA from sperm smear.
- Author:
Yao-dong SUN
1
;
Hong REN
;
Yue-yong CHEN
Author Information
1. Institute of Criminal Science,Public Security Bureau of Yancheng City, Yancheng 224002, China.
- Publication Type:Journal Article
- MeSH:
Adult;
DNA/isolation & purification*;
DNA Fingerprinting/methods*;
Female;
Forensic Medicine;
Humans;
Male;
Polymerase Chain Reaction;
Rape;
Specimen Handling;
Spermatozoa/chemistry*;
Tandem Repeat Sequences/genetics*
- From:
Journal of Forensic Medicine
2004;20(4):202-204
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an effective method for testing DNA from the sperm smear.
METHODS:We did exploratory research for ninety-one cases of the sperm smear which were accumulated by our laboratory during working hours. Chelex extraction method was used to extract DNA. The digested solution of sperm was purified and concentrated by using Silicon bead. STR loci were typed after PCR amplification by Profile Plus kit.
RESULTS:Although there were a few sperm cells on the slide, it is easy to obtain good DNA typing result from it because the amalgamative method we used was high effective for DNA extraction.
CONCLUSION:The DNA analysis of sperm smear could offer satisfactory typing results which would be useful in case.
