The study on HLA-Cw polymorphism from Xi'an Han population by PCR-sequence specific oligonucleotide probe.
- Author:
Hong-bo ZHANG
1
;
Jiang-hua LAI
;
Jun-hai ZHAO
;
Shengbin LI
Author Information
1. The National Laboratory of the Ministry of Health for Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China. zhanghb@mail.xjtu.edu.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
Asian People/genetics*;
China/ethnology*;
Gene Frequency;
Genotype;
HLA-C Antigens/genetics*;
Humans;
Oligonucleotide Probes;
Polymerase Chain Reaction/methods*;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2004;20(4):197-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the HLA-Cw allele and genotype frequencies from Xi'an Han population and obtain genetic data.
METHODS:The results of HLA-Cw typing for 130 randomly selected from Xi'an Han population were obtained by using the PCR-Sequence Specific Oligonucleotide Probes (SSOP).
RESULTS:In this investigation, 16 alleles were detected among 130 unrelated individuals with frequencies from 0.0077 to 0.1588 and HLA-Cw*01,03,07 were the most common HLA-Cw alleles. We have made a survey of HLA-Cw alleles frequencies in Xi'an Han Population, with blank frequency being lowered to 0.018 2.
CONCLUSION:The distribution of genotype frequencies met the law of Hardy-Weinberg equilibrium by Hi-square test. The frequency data can be used in forensic and paternity tests, transplant matching and anthropology.
