ABO genotyping by duplex amplification and oligonucleotide arrays assay.
- Author:
Li LI
1
;
Cheng-tao LI
;
Rong-yu LI
;
Meiqian SUN
;
Yan LIU
;
Yao LI
;
Yuan LIN
;
Tingzhi QUE
;
Dalin CHENG
;
Pinhua YAN
;
Jianxin FANG
;
Zhenmin ZHAO
;
Min SHEN
;
Zhichun DU
Author Information
1. Institute of Forensic Sciences, Ministry of Justice, PRChina, Shanghai 200063, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
ABO Blood-Group System/genetics*;
Blood Stains;
DNA/blood*;
DNA Primers;
Female;
Forensic Medicine;
Genotype;
Hair/chemistry*;
Humans;
Oligonucleotide Array Sequence Analysis
- From:
Journal of Forensic Medicine
2004;20(4):193-196
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:ABO genotyping for forensic identification by oligonucleotide chip.
METHODS:Oligonucleotide microarrays which could detect 3 different SNPs in exon 6 and exon 7 for ABO genotyping were used. Population studies on ABO was carried out in a sample of 115 unrelated Chinese Han individuals. The method was also applied to cases.
RESULTS:The technique could identify 6 genotypes of ABO system. According to the results of population studies, no significant deviations from Hardy-Weinberg equilibrium could be found. The observed and expected heterozygosities were 0.591 and 0.616 respectively. The polymorphic information content was 0.544. The average exclusion probabilities in buos and trios was 0.188 and 0.344 respectively. The discrimination power is 0.777.
CONCLUSION:The data and case application demonstrated that ABO typing by oligonucleotide probe arrays was a useful technique for paternity testing and individual identification.
