A study of genetic polymorphism of the STR locus D7S2846, D19S400 and D18S535 in Han population in Wenzhou.
- Author:
Hong-qin ZHANG
1
;
Shu-zhen WU
;
Yun-tian BI
Author Information
1. Molecular Biology Research Centre of Wenzhou Medical College, Wenzhou 325027, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
China/ethnology*;
DNA/isolation & purification*;
Gene Frequency;
Genotype;
Humans;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2004;20(3):160-161
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study genetic polymorphism of D7S2846, D19S400 and D18S535 in Han population in Wenzhou.
METHODS:DNA was extracted with chelex-100 method from EDTA-blood samples of 194 unrelated individuals in Wenzhou and amplified with PCR technique. The PCR products were analyzed by PAG vertical electrophoresis and silver-stain.
RESULTS:6 alleles and 15 genotypes of D7S2846, 10 alleles and 36 genotypes of D19S400, 8 alleles and 26 genotypes of D18S535 was observed. The heterozygosities of the three loci are 0.644, 0.724 and 0.772; The power of discriminating are 0.854, 0.940 and 0.938.
CONCLUSION:The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium (P>0.05), so the three loci can be used in forensic medicine and in other genetic researches.
