Study of DNA identification in burned bones.
- Author:
Jian YE
1
;
An-quan JI
;
Xing-chun ZHAO
Author Information
1. Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. yejian_21@sina.com.cn
- Publication Type:Journal Article
- MeSH:
Bone and Bones/chemistry*;
Burns/metabolism*;
Cetrimonium Compounds;
DNA/isolation & purification*;
DNA Fingerprinting/methods*;
Female;
Forensic Medicine/methods*;
Humans;
Male;
Polymerase Chain Reaction/methods*;
Sensitivity and Specificity;
Tandem Repeat Sequences;
Time Factors
- From:
Journal of Forensic Medicine
2004;20(3):155-159
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:For the purpose of solving a problems of DNA testing of burned bones.
METHODS:We present a novel strategy to obtain DNA from burned bones based on the use of cetyltrimethylammonium bromide (CTAB) lysis buffer and isoamyl alcohol-chlorophorm extraction with subsequent DNA purification using the DNA IQ System.
RESULTS:The methods were found to be effective in removing the PCR inhibitors from the burned bone. Then the extracted DNA was successfully genotyped by using the florescence labeling STR multiplex method.
CONCLUSION:The results of this research will assist forensic scientists in the identification of DNA from victims whose bodies underwent significant trauma or burning, precluding the utilization of traditional forensic DNA identification techniques.
