Study on relationship between changes of geometry parameters in human spleen nuclei and the postmortem interval.
- Author:
Xi-ji SHU
1
;
Li-jiang LIU
;
Jia-wei HU
Author Information
1. School of Medicine & Life Sciences, Jianghan University, Wuhan 430056, China. shuxiji@sina.com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Adolescent;
Adult;
Cell Nucleus/ultrastructure*;
Cytophotometry/methods*;
DNA/metabolism*;
Female;
Forensic Pathology;
Humans;
Image Processing, Computer-Assisted/methods*;
Male;
Middle Aged;
Spleen/metabolism*;
Time Factors
- From:
Journal of Forensic Medicine
2004;20(3):140-142
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Using computer image-analyze technique (CIAT) to study changes of geometry parameters in human spleen nuclei and seek a new experimental method to deduce the estimation the postmortem interval (PMI).
METHODS:31 cadavers that known accurate PMI, sampled and smeared respectively every hour within the first 36 hours after death, fixed with cold Carony fixation, stained by Feulgen-van's method, and measured 5 geometry parameters using the image-analyze instrument including Area (A), Mean-Dia (MD), Average Diameter (AD), perimeter (P), Index of density (ID).
RESULTS:A, MD, AD and P in the human spleen nuclei have no correlation with the PMI. But ID rose regularly with the prolongation of PMI in 36 hours. There was a definite correlation between ID and the PMI, r=0.983, linear regression equation with PMI (hours) as the dependent variable was calculated for ID.
CONCLUSION:Geometry parameter ID was proved to be preferable indexes for estimation of PMI in 36 hours.
