Polymorphism of STR loci D12S391/D18S865 in Wenzhou Han population.
- Author:
Shu-zhen WU
1
;
Hong-qin ZHANG
;
Yun-tian BI
Author Information
1. The Molecular Biology Research Center of Wenzhou Medical College, WenZhou 325027, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
China/ethnology*;
DNA/isolation & purification*;
Electrophoresis, Polyacrylamide Gel;
Forensic Medicine;
Gene Frequency;
Genotype;
Humans;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2004;20(2):85-87
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the polymorphism and forensic efficiency values of STR loci D12S391/D18S865
METHODS:Population studies on D12S391 and D18S865 were carried out in a sample of 454 unrelated Wenzhou Han individuals using PCR followed by PAGE and silver stain detection.
RESULTS:11/7 alleles and 50/21 genotypes of D12S391/D18S865 were respectively observed among the 454 unrelated individuals. The genotype frequency matched the Hardy-Weinberg equilibrium, The heterozygotes (H) in D12S391/D18S865 were 0.7974/0.7379, Power of Exclusion were 0.9566/0.9077, polymorphism information content (PIC) were 0.8260/0.7279 respectively.
CONCLUSION:D12S391 and D18S865 were high polymorphic loci and the frequencies were in accordance with Hardy-Weinberg equilibrium (P>0.5), so the two loci can be used in forensic medicine and in other genetic research areas.
