The study on expression of NF-kappaB in experimental brain contusion in rats.
- Author:
Lu-yang TAO
1
;
Xi-ping CHEN
;
Mei DING
Author Information
1. School of Medicine, Suzhou University, Suzhou 215007, China. luyangtao@suda.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Blotting, Western;
Brain Injuries/pathology*;
Immunohistochemistry;
Injury Severity Score;
NF-kappa B/genetics*;
RNA, Messenger/biosynthesis*;
Rats;
Reverse Transcriptase Polymerase Chain Reaction;
Staining and Labeling;
Time Factors
- From:
Journal of Forensic Medicine
2004;20(2):73-80
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of NF-kappaB in different post-traumatic intervals and severity of brain injury.
METHODS:Fifty-four brain tissue samples of slight (n=24), moderate (n=24) brain injury, sham (n=3) and control (n=3) of rat were examined by immunohistochemical staining, westernblot and RT-PCR.
RESULTS:Up-regulating of NF-kappaB expression was found in tissues from traumatic brain injury compared with controls in early 1 hour after TBI, and reached peak at 24h and 48h and disappeared after 7 days. The expression of NF-kappaB mRNA has association with contusion severity.
CONCLUSION:NF-kappaB may benefit to the estimation of posttraumatic intervals of brain injury.
