Investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells of rat.
- Author:
Zhi-ping LIU
1
;
Xin CHEN
;
Yi-wen SHE
Author Information
1. Department of Forensic Medicine, Medical Center of Fudan University, Shanghai 200032, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Cell Nucleus/metabolism*;
DNA/metabolism*;
Flow Cytometry;
Kidney/metabolism*;
Liver/metabolism*;
Postmortem Changes;
Rats;
Rats, Sprague-Dawley;
Rosaniline Dyes;
Spleen/metabolism*;
Time Factors
- From:
Journal of Forensic Medicine
2004;20(2):68-69
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells.
METHODS:After different PMI from the heart, liver, spleen and kidney were taken into pieces respectively, then centrifuged and digested to get suspending cells fluid. The amount of DNA of rats'viscera were detected by flow cytometry after stained by fluorescence, and also inspect the amount of DNA in different periods to find out the law of its variation.
RESULTS:It showed a descendent trend of the amount of DNA in cells after different PMI, especially in spleen.
CONCLUSION:The amount of DNA of all the viscera grows downwards after death, this might be applyed in forensic estimation of PMI.
