The study on the expression of caspase-3 in experimental brain contusion in rats.
- Author:
Lu-yang TAO
1
;
Xi-ping CHEN
;
Shi-zhong BIAN
Author Information
1. School of Medicine, Suzhou University, Suzhou 215007, China. luyangtao@suda.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Blotting, Western;
Brain Injuries/pathology*;
Caspase 3;
Caspases/genetics*;
Immunohistochemistry;
RNA, Messenger/biosynthesis*;
Rats;
Reverse Transcriptase Polymerase Chain Reaction;
Time Factors
- From:
Journal of Forensic Medicine
2004;20(1):9-12
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of caspase-3 in different posttraumatic intervals and severity of brain injury.
METHODS:The study examined brain tissue samples of slight (n = 24), severe (n = 24) brain injury and control (n = 6) of rat, using immunohistochemical staining, western-blot and RT-PCR method.
RESULTS:Up-regulating of caspase-3 expression was found in tissue from traumatic brain injury compared with controls in early 1 hour after TBI, and lasted for 14 days. The gray degree and threshold area of caspase-3 positive cells is different in different severity of brain injury.
CONCLUSION:The increasing of caspase-3 expression indicates that TBI exists. The gray degree, threshold area of caspase-3 positive cells and the cleavage degree of pro-caspase-3 have association with the severity of brain injury.
