DNA analysis of a 500 year mummy sample.
- Author:
Da-yue TONG
1
;
Xin-yao WU
;
Gui-qing CAI
Author Information
1. Faculty of Forensic Medicine, Zhongshan Medical College, Zhongshan University, Guangzhou 510089, China. tddy0@163.com
- Publication Type:Journal Article
- MeSH:
Base Sequence;
DNA/genetics*;
DNA, Mitochondrial/genetics*;
Forensic Medicine;
Humans;
Microsatellite Repeats/genetics*;
Molecular Sequence Data;
Mummies;
Sequence Analysis, DNA/methods*
- From:
Journal of Forensic Medicine
2003;19(4):199-200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To accumulate experience for dated forensic matter analysis, for example, Mummy.
METHODS:DNA are extracted by methods of phenol-chloroform and are purified by Wizard DNA clean-up system. The STRs locus are ampolification with Promega Powerplus 16 system. The mtDNA hypervariable region 1 (HV1) is amplificated by '3 pair primers'. The products were sequenced with 377 DNA sequencer.
RESULTS:The STRs locus very distinctness and mtDNA sequence is correct.
CONCLUSION:It is a valuable method for special forensic matters.
