Seven Y-specific loci haplotypes and their usefulness in forensic casework.
- Author:
Qiu-ling LIU
1
;
De-jian LU
;
Li-xian CHEN
Author Information
1. Faulty of Forensic Medicine, Zhong Shan Medical College, Zhong Shan University, Guangzhou 510089, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian People;
Chromosomes, Human, Y/genetics*;
Forensic Medicine;
Gene Frequency;
Genetic Markers;
Genetics, Population;
Genotype;
Haplotypes;
Humans;
Male;
Microsatellite Repeats/genetics*;
Paternity;
Polymerase Chain Reaction;
Polymorphism, Genetic;
Sequence Analysis, DNA
- From:
Journal of Forensic Medicine
2003;19(4):196-200
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study genetic polymorphism of 7 Y-specific short tandem repeats (STR) and assess their usefulness in forensic casework.
METHODS:7 Y-STR have been amplified in two multiplex reactions, (Multiplex I:DYS391, GATA-A4, GATA-A10 and GATA-H4. Multiplex II:DYS439,DYS437 and DYS434). PCR products were separated by polyacrylamide gels electrophoresis followed by silver stain.
RESULTS:When 372 unrelated individuals from the Han population in Guangdong were detected by those system, DYS391, GATA-A4, GATA-A10, GATA-H4,DYS439,DYS437 and DYS434 showed 5,7,6,5,6,4,4 alleles, respectively. A total of 254 different haplotypes were identified, of which 201 (79.13%) were found in single individuals. The overall haplotypes diversity reached 0.9960.
CONCLUSION:The 7 Y-STR loci are highly genetic polymorphism and they will be very powerful for establishing Y-STR database, understanding human origin, paternity testing and personal identification.
