Genotype reliability of short tandem repeats typing from minute DNA.
- Author:
De-jian LU
1
;
Hong-yu SUN
;
Li-xian CHEN
Author Information
1. Department of Forensic Biology, Zhongshang Medical College, Guangzhou 510089, China. dejian6182@sina.com
- Publication Type:Journal Article
- MeSH:
Alleles;
DNA/genetics*;
Forensic Medicine;
Genotype;
Humans;
Male;
Minisatellite Repeats/genetics*;
Sequence Analysis, DNA;
Tandem Repeat Sequences/genetics*
- From:
Journal of Forensic Medicine
2003;19(3):151-153
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To understand the forensic practice of DNA profiling from minimal amount of DNA.
METHODS:Serial dilutions of DNA were amplified with the PowerPlex 16 System Kit, then the genotyping of short tandem repeat(STR) was performed by ABI 377 DNA automated Sequencer.
RESULTS:When the mount of DNA template was less than 250 pg, allelic drop-out apparently occurred at several loci. Other disturbed peaks, such as artefact bands and imbalanced heterozygote, also presented.
CONCLUSION:The anomalous results may result in incorrect genotyping. Careful and comprehensive considerations are needed to interpret the STR profile of minute DNA.
