Factors affecting the determination of the percent carboxyhemoglobin saturation of blood.
- Author:
Yan JIANG
1
;
Yong-hong YE
;
Yi-fan ZHANG
Author Information
1. Department of Forensic Medicine, Medical Center of Fudan University, Shanghai 200032, China.
- Publication Type:Journal Article
- MeSH:
Carbon Monoxide Poisoning/diagnosis*;
Carboxyhemoglobin/analysis*;
Forensic Medicine;
Humans;
Spectrophotometry/methods*;
Temperature
- From:
Journal of Forensic Medicine
2003;19(2):88-91
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate factors affecting the determination of the percent carboxyhemoglobin saturation (HbCO%) of blood in an attempt to offer further data for results interpretation and sample storage requirement.
METHODS:The HbCO% of blood samples stored in various conditions were detected by three spectrophotometries during the succeeding 30 days.
RESULTS:The data detected by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry were more stable than mono-wavelength spectrophotometry. The HbCO% of blood was significantly related with the storage conditions which include temperature, time and the degree of exposure to air.
CONCLUSION:Determinations of HbCO% are reliable which performed by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry, combine with spectral scans. During 30 days, blood stored at 4 degrees C exposed to a limited volume of air does not influence the determination of HbCO%.
