A study on the relationship between the expression of apoptosis-related gene and the injury time in earlier period following cerebral contusion.
- Author:
Xu-dong SONG
1
;
Wei-dong SHI
;
Qi-sheng QIN
Author Information
1. Department of Forensic Pathology, Tongji Medical College of Huazhong, University of Science and Technology, Wuhan 430030, China. Songxd2002@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis/genetics*;
Brain/pathology*;
Brain Concussion/pathology*;
Female;
Forensic Medicine;
Male;
Proto-Oncogene Proteins c-bcl-2/genetics*;
Rats;
Rats, Wistar;
Time Factors;
fas Receptor/genetics*
- From:
Journal of Forensic Medicine
2003;19(2):78-80
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between the expressive variety of apoptosis-related gene Bcl-2, Fas and the brain injury time during earlier period after cerebral contusion.
METHODS:The morphologia feature of brain tissue was observed under microscopy and immunohistochemistry, image analyze technique was applied to detected the expression of Bcl-2 and Fas.
RESULTS:Typical apoptotic cells were seen 8 hours after cerebral contusion. The expression of apoptosis-related gene was noted after 30 minutes and keep increase subsequently. The expression of Bcl-2 reached maximum level at 4 hours after brain trauma and then declined.
CONCLUSION:Detecting the expression of Bcl-2 and Fas would be of valuable in the distinguishment of promortem and postmortem injury, and the estimation of brain injury time during the earlier period after cerebral contusion.
