Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats.
- Author:
Meng-yun MA
1
;
Xiaohu XU
Author Information
1. Shenzhen Criminal Science & Technology Institute, Shenzhen 518008, China.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Female;
Forensic Medicine;
In Situ Hybridization;
Male;
Myocardial Reperfusion Injury/metabolism*;
Myocytes, Cardiac/metabolism*;
Proto-Oncogene Proteins c-fos/genetics*;
RNA, Messenger/genetics*;
Random Allocation;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Forensic Medicine
2003;19(2):65-67
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/R) during acute period.
METHODS:The model of I/R was established, and the rats was divided normal control, ischmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes.
RESULTS:After 10 min ischemia and 30 min reperfusion, the area of reperfusion showed a part of cardiac myocytes weak staining, and get to peak at I-60 min/R-30 min. The myocardium in normal control and ischemia groups less than 60 min showed negative staining. No changes were found in all groups by HE staining.
CONCLUSION:c-fos mRNA detection may become an important method for diagnosis of I/R.
