New improved approaches for DYS385 detection.
- Author:
Shuang-bo TANG
1
;
Jing-yuan GUO
;
Zhao-hui LI
Author Information
1. Faculity of Forensic Medicine, Sun Yat-sen University, Guangzhou 510089. tangshuangbo@163.net
- Publication Type:Journal Article
- MeSH:
Chromosomes, Human, Y/genetics*;
Forensic Medicine;
Gene Frequency;
Humans;
Male;
Polymerase Chain Reaction/methods*;
Sequence Analysis, DNA/methods*;
Tandem Repeat Sequences
- From:
Journal of Forensic Medicine
2003;19(1):27-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish more sensitive methods for detection of DYS385 in routine forensic casework.
METHODS:The primers recommended by Genome Database (GDB) and Schneider were used to amplify DYS385 respectively. Then, a semi-nested PCR of DYS385 was designed by using the two different primers as outer and inner primer. A series of experiments were carried out to achieve good result by adjusting the ratio of outer/inner primer and optimizing the PCR condition.
RESULTS:It showed that an overall 112 bp shorter DYS385 fragments and better electrophoretic separation were obtained by using primer2B. By using the semi-nested PCR approach, the shorter specific DYS385 fragments could be amplified and detectable DNA amounted to 50 pg.
CONCLUSION:This method is 20 fold more sensitive than the ordinary method.
