A analysis of ELISA on the time-related expression of IL-2 and TNF-alpha during the healing process of wound in rat skin.
- Author:
Hui-jun WANG
1
;
Yun-chuan DING
Author Information
1. Department of Pathology, First Military Medical University, Guangzhou 510515.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Enzyme-Linked Immunosorbent Assay;
Female;
Interleukin-2/metabolism*;
Male;
Rats;
Rats, Wistar;
Skin/metabolism*;
Time Factors;
Tumor Necrosis Factor-alpha/metabolism*;
Wound Healing/physiology*
- From:
Journal of Forensic Medicine
2003;19(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:In order to explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound.
METHODS:A assay of ELISA were performed on intravital skin wounds(after incision 0.5-168 h) to detect the dynamics expression level of IL-2, TNF-alpha.
RESULTS:The level of IL-2 and TNF-alpha increased at 0.5 h after wounding, then got to a peak at 3 h and 1 h after injure respectively. Rebound of TNF and IL-2 levels were shown at 48 h after wounding, and both cytokine levels were inclined to elevating between 72 h and 168 h after wounding.
CONCLUSION:The cytokine level changes suggest they have a time-related expression during wounds healing process.
