The study on expression of caspase-1 in experimental brain contusion of different severity in rats.
- Author:
Lu-yang TAO
1
;
Xi-ping CHEN
;
Mei DING
Author Information
1. China Medical University, School of Forensic Medicine, Shenyang, P.R. China 110001. luyangtao@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Brain/metabolism*;
Brain Injuries/metabolism*;
Caspase 1/metabolism*;
Female;
Injury Severity Score;
Male;
RNA, Messenger/metabolism*;
Rats;
Rats, Wistar;
Time Factors
- From:
Journal of Forensic Medicine
2003;19(1):4-7
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of caspase-1 in different posttraumatic intervals and severity of brain injury.
METHODS:The study examined brain tissue samples of slight (n = 24), severe (n = 24) brain injury and control (n = 6) of rat, using immunohistochemical staining, western-blot and RT-PCR method.
RESULTS:Up-regulating of caspase-1 expression was found in tissue from traumatic brain injury compared with controls in early 1 hour after TBI, and lasted for 2 weeks. Caspase-1 cleaved after brain injury.
CONCLUSION:These findings are the first time to demonstrate the study of different intense brain injury. The increasing of caspase-1 expression indicates that the TBI exists. The threshold area of caspase-1 positive cells is different in different severity of brain injury.
