PGM1 genotyping by PCR-SSCP.
- Author:
Hai-yan SONG
1
;
Qing-en YANG
;
Chun-ying YU
Author Information
1. Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Alleles;
Asian People/genetics*;
China;
DNA/genetics*;
Gene Frequency;
Genotype;
Humans;
Phosphoglucomutase/genetics*;
Polymerase Chain Reaction/methods*;
Polymorphism, Genetic;
Polymorphism, Single-Stranded Conformational;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2002;18(3):152-154
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:PGM1 genotyping by PCR-SSCP analysis.
METHODS:Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes.
RESULTS:2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318. PCR-SSCP analysis was suitable for determination of PGM1 genotypes from old blood and semen stains.
CONCLUSION:PGM1 system typed by PCR-SSCP is useful for forensic identification.
