Expression of EIIIA-fibronectin in injured rat skin used in estimation of wound interval.
- Author:
Ning-guo LIU
1
;
Yi-jiu CHEN
;
Xiao-hua HUANG
Author Information
1. Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063. liuningguo@163.net
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alternative Splicing;
Animals;
Epithelium/metabolism*;
Fibronectins/genetics*;
Forensic Medicine;
Integrin alpha4beta1/biosynthesis*;
RNA, Messenger/metabolism*;
Rats;
Reverse Transcriptase Polymerase Chain Reaction;
Skin/metabolism*;
Time Factors
- From:
Journal of Forensic Medicine
2002;18(3):129-131
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the means of fibronectin(FN) alternative splicing and the expression of EIIIA-FN variant in rat skin after bruise, for the sake of providing some help for forensic estimation of wound interval.
METHODS:Total RNA was isolated from wounded skin, and reverse transcription polymerase chain reaction was conducted to amplify target segments.
RESULTS:Detectable EIIIA+(526 bp) segments, lacked in normal organize, was amplified at 1 h after experimental wound, and the levels were increased within 24 h.
CONCLUSION:The alternative splicing EIIIA-fibronectin variant would be a satisfied criterion for research of skin injury.
