Column-switching high-performance liquid chromatographic method for the determination of morphine and O6-monoacetylmorphine in urine.
- Author:
Yu-rong ZHANG
1
;
Rong WANG
;
Cheng-gong ZHANG
Author Information
1. Shanghai Institute of Forensic Science, Shanghai 200083.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Heroin Dependence/urine*;
Humans;
Morphine/urine*;
Morphine Derivatives/urine*;
Sensitivity and Specificity
- From:
Journal of Forensic Medicine
2002;18(2):89-91
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a column-switching high-performance liquid chromatographic method for the determination of morphine and O6-monoacetylmorphine in urine.
METHODS:Urine samples (1.0 ml) were spiked with 1.0 ml borate buffer, after centrifugation, 1.0 ml of supernate were injected directly into an extraction column (YWG C18 33 mm x 5.0 mm, 10 microns). After a washing step with the extraction mobile phase, the retained morphine and O6-monoacetylmorphine were flushed into the analytical column (Lichrospher 100 CN 125 mm x 4.0 mm, 5 microns) with the mobile phase CH3OH-H2O (60:40). The analytical mobile phase is CH3OH-phosphate buffer (pH6.86) (22:78). The UV detector was set at lambda 286 nm.
RESULTS:The method shows excellent linearity from 50 to 1,600 ng/ml for morphine and from 100 to 1,600 ng/ml for O6-monoacetylmorphine. The linear correlation coefficients were > 0.999. The relative standard deviations were < 4%. The limits of detection were 40 ng/ml for both morphine and O6-monoacetylmorphine.
CONCLUSION:The method described is sensitive, rapid, reproducible, and simple.
