The in situ hybridization effort estimation between antisense RNA probe and random primed DNA probe.
- Author:
Ning-guo LIU
1
;
Zi-qin ZHAO
;
Yun-ju GU
Author Information
1. Institution of Forensic Sciences of Ministry of Justice, Shanghai 200063. liuningguo@163.net
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
DNA, Antisense;
Digoxigenin;
Fibronectins/genetics*;
In Situ Hybridization/methods*;
RNA Probes;
RNA, Messenger/biosynthesis*;
Random Amplified Polymorphic DNA Technique/methods*;
Rats;
Rats, Sprague-Dawley;
Skin/pathology*
- From:
Journal of Forensic Medicine
2002;18(1):7-11
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To estimate the effort between antisense RNA probe and random primed DNA probe.
METHODS:The in situ hybridization was conducted on parafin section from wounding model of rat skin.
RESULTS:Although both probes appeared positive staining, RNA probes was superior to DNA probes in terms of depth of staining and background.
CONCLUSION:RNA probe showed more satisfactorily on ISH.
