Cell-derived nanovesicles from mesenchymal stem cells as extracellular vesicle-mimetics in wound healing.
10.1016/j.apsb.2022.10.022
- Author:
Yub Raj NEUPANE
1
;
Harish K HANDRAL
2
;
Syed Abdullah ALKAFF
3
;
Wei Heng CHNG
1
;
Gopalakrishnan VENKATESAN
1
;
Chenyuan HUANG
4
;
Choon Keong LEE
1
;
Jiong-Wei WANG
4
;
Gopu SRIRAM
5
;
Rhonnie Austria DIENZO
6
;
Wen Feng LU
2
;
Yusuf ALI
6
;
Bertrand CZARNY
3
;
Giorgia PASTORIN
1
Author Information
1. Department of Pharmacy, National University of Singapore, Singapore 117559, Singapore.
2. Department of Mechanical Engineering, National University of Singapore, Singapore 117575, Singapore.
3. School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
4. Department of Surgery, National University of Singapore, Singapore 119228, Singapore.
5. Faculty of Dentistry, National University of Singapore, Singapore 119085, Singapore.
6. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 639798, Singapore.
- Publication Type:Journal Article
- Keywords:
Bionanotechnology;
Cell migration;
Cell proliferation;
Cell-derived nanovesicles;
ECM;
Extracellular vesicles;
Fibroblasts;
Mesenchymal stem cells;
Wound healing
- From:
Acta Pharmaceutica Sinica B
2023;13(5):1887-1902
- CountryChina
- Language:English
-
Abstract:
Wound healing is a dynamic process that involves a series of molecular and cellular events aimed at replacing devitalized and missing cellular components and/or tissue layers. Recently, extracellular vesicles (EVs), naturally cell-secreted lipid membrane-bound vesicles laden with biological cargos including proteins, lipids, and nucleic acids, have drawn wide attention due to their ability to promote wound healing and tissue regeneration. However, current exploitation of EVs as therapeutic agents is limited by their low isolation yields and tedious isolation processes. To circumvent these challenges, bioinspired cell-derived nanovesicles (CDNs) that mimic EVs were obtained by shearing mesenchymal stem cells (MSCs) through membranes with different pore sizes. Physical characterisations and high-throughput proteomics confirmed that MSC-CDNs mimicked MSC-EVs. Moreover, these MSC-CDNs were efficiently uptaken by human dermal fibroblasts and demonstrated a dose-dependent activation of MAPK signalling pathway, resulting in enhancement of cell proliferation, cell migration, secretion of growth factors and extracellular matrix proteins, which all promoted tissue regeneration. Of note, MSC-CDNs enhanced angiogenesis in human dermal microvascular endothelial cells in a 3D PEG-fibrin scaffold and animal model, accelerating wound healing in vitro and in vivo. These findings suggest that MSC-CDNs could replace both whole cells and EVs in promoting wound healing and tissue regeneration.
