Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea.
10.3347/kjp.2003.41.3.147
- Author:
Jong Yil CHAI
;
Aifen LIN
;
Eun Hee SHIN
;
Myoung Don OH
;
Eun Taek HAN
;
Ho Woo NAN
;
Soon Hyung LEE
- Publication Type:Case Reports ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii Korean Isolate-1 (KI-1) and RH strain;
ocular patient;
immunoblotting;
culture (in vitro);
passage (in mice)
- MeSH:
Animals;
Antigens, Protozoan/analysis;
Female;
Humans;
Korea;
Mice;
Mice, Inbred BALB C;
Microscopy, Electron;
Middle Aged;
Parasitemia/parasitology;
Sarcoma 180;
Serial Passage;
Specific Pathogen-Free Organisms;
*Toxoplasma/classification/growth & development/isolation & purification/pathogenicity;
Toxoplasmosis, Ocular/*diagnosis/parasitology;
Tumor Cells, Cultured;
Virulence
- From:The Korean Journal of Parasitology
2003;41(3):147-154
- CountryRepublic of Korea
- Language:English
-
Abstract:
Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a wellknown virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.
