Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda) .
10.3347/kjp.2003.41.3.155
- Author:
Min Ho CHOI
;
Jin Sook RYU
;
Me Jeong LEE
;
Shunyu LI
;
Byung Suk CHUNG
;
Jong Yil CHAI
;
Paiboon SITHITHAWORN
;
Smarn TESANA
;
Sung Tae HONG
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Clonorchis sinensis;
Opisthorchis viverrini;
antigens;
diagnosis
- MeSH:
Animals;
Antigens, Helminth/analysis/*chemistry/immunology;
Clonorchiasis/*diagnosis;
Clonorchis sinensis/*immunology;
Cross Reactions;
Electrophoresis, Polyacrylamide Gel/veterinary;
Humans;
Immunoblotting/veterinary;
Molecular Weight;
Opisthorchiasis/*diagnosis;
Opisthorchis/*immunology
- From:The Korean Journal of Parasitology
2003;41(3):155-163
- CountryRepublic of Korea
- Language:English
-
Abstract:
The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.
