Mechanism of Der f 1/IGF-1 nanoparticle promoting the production of regulatory T cell.
10.13201/j.issn.2096-7993.2023.04.007
- Author:
Longpeng MA
1
;
Xiangqian LUO
1
;
Lihua MO
1
;
Jialiang FAN
1
;
Dabo LIU
1
Author Information
1. Department of Pediatric Otolaryngology，Shenzhen Hospital，Southern Medical University，Shenzhen，518060，China.
- Publication Type:Journal Article
- Keywords:
DC cells;
nanoparticles;
regulatory T cell
- MeSH:
Humans;
T-Lymphocytes, Regulatory/metabolism*;
Interleukin-10/metabolism*;
Insulin-Like Growth Factor I;
Transforming Growth Factor beta;
Nanoparticles/chemistry*;
Particle Size;
Drug Carriers/chemistry*
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2023;37(4):272-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare PLGA nanoparticles loaded with Der f 1/IGF-1（Der f 1/IGF-1 NPs） and investigate their role in promoting the formation of Treg cells. Methods:NPs coated with Der f 1/IGF-1 were prepared by double emulsion method and their physicochemical properties and cumulative release rate in vitro were analyzed. After pretreatment, BMDC was divided into Saline group, Blank NPs group, Der f 1/IGF-1 group and Der f 1/IGF-1 NPs group. Determination of the expression of IL-10 and TGF-β in BMDC by ELISA. The number of Treg cells was detected by flow cytometry. Results:The results showed that Der f 1/IGF-1 NPs were spherical structures, with good dispersion, particle size less than 200 nm, negative charge and stable slow-release effect of Zeta potential. After BMDC pretreatment, the expression levels of TGF-β and IL-10 in BMDC cells in the Der f 1/IGF-1 NPs group were significantly increased compared with the Blank NPs group, and the difference was statistically significant（P<0.001）. After co-culture with CD4+ T cells, the proportion of Treg cells produced in the Der f 1/IGF-1 NPs group was significantly increased, and the difference was statistically significant（P<0.001）. Conclusion:Der f 1/IGF-1 NPs can induce Treg cell generation in vitro. This study provides a new and more effective method for the reconstruction of immune tolerance dysfunction.
