Lysohexaenetides A and B, linear lipopeptides from Lysobacter sp. DSM 3655 identified by heterologous expression in Streptomyces.
10.1016/S1875-5364(23)60473-X
- Author:
Qiushuang XU
1
,
2
;
Haochen ZOU
3
;
Chen PAN
3
;
Haoxin WANG
4
;
Yuemao SHEN
3
;
Yaoyao LI
5
Author Information
1. Key Laboratory of Chemical Biology of Ministry of Education, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China
2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
3. Key Laboratory of Chemical Biology of Ministry of Education, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
4. State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
5. Key Laboratory of Chemical Biology of Ministry of Education, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China. Electronic address: liyaoyao@sdu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Heterologous expression;
Lipopeptides;
Lysobacter;
Natural products
- MeSH:
Lysobacter/metabolism*;
Streptomyces/metabolism*;
Lipopeptides/metabolism*;
Polyketide Synthases/genetics*;
Multigene Family
- From:
Chinese Journal of Natural Medicines (English Ed.)
2023;21(6):454-458
- CountryChina
- Language:English
-
Abstract:
Lysobacter harbors a plethora of cryptic biosynthetic gene clusters (BGCs), albeit only a limited number have been analyzed to date. In this study, we described the activation of a cryptic polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) gene cluster (lsh) in Lysobacter sp. DSM 3655 through promoter engineering and heterologous expression in Streptomyces sp. S001. As a result of this methodology, we were able to isolate two novel linear lipopeptides, lysohexaenetides A (1) and B (2), from the recombinant strain S001-lsh. Furthermore, we proposed the biosynthetic pathway for lysohexaenetides and identified LshA as another example of entirely iterative bacterial PKSs. This study highlights the potential of heterologous expression systems in uncovering cryptic biosynthetic pathways in Lysobacter genomes, particularly in the absence of genetic manipulation tools.